Physics Colloquium: MINFLUX nanoscopy and related matters

Prof. Stefan W. Hell, Max Planck Institute for Biophysical Chemistry, Göttingen & Max Planck Institute for Medical Research, Heidelberg

22 March 2021, 16:00 
Zoom: https://weizmann.zoom.us/j/95175122285?pwd=RUpiUEYzeFBmdlovbkxHZW94aTJVUT09 
Physics Colloquium

Zoom: https://weizmann.zoom.us/j/95175122285?pwd=RUpiUEYzeFBmdlovbkxHZW94aTJVUT09

 

Abstract: 

I will show how an in-depth description of the basic principles of diffraction-unlimited fluorescence microscopy (nanoscopy) [1-3] has spawned a new powerful superresolution concept, namely MINFLUX nanoscopy [4]. MINFLUX utilizes a local excitation intensity minimum (of a doughnut or a standing wave) that is targeted like a probe in order to localize the fluorescent molecule to be registered. In combination with single-molecule switching for sequential registration, MINFLUX [4-6] has obtained the ultimate (super)resolution: the size of a molecule. MINFLUX nanoscopy, providing 1–3 nanometer resolution in fixed and living cells, is presently being established for routine fluorescence imaging at the highest, molecular-size resolution levels. Relying on fewer detected photons than popular camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells.
 
 
[1] Hell, S.W., Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission- depletion fluorescence microscopy. Opt. Lett. 19, 780-782 (1994).
[2] Hell, S.W. Far-Field Optical Nanoscopy. Science 316, 1153-1158 (2007).
[3] Hell, S.W. Microscopy and its focal switch. Nat. Methods 6, 24-32 (2009). 
[4] Balzarotti, F., Eilers, Y., Gwosch, K. C., Gynnå, A. H., Westphal, V., Stefani, F. D., Elf, J., Hell, S.W. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science 355, 606-612 (2017). 
[5] Eilers, Y., Ta, H., Gwosch, K. C., Balzarotti, F., Hell, S. W. MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution. PNAS 115, 6117-6122 (2018).
[6] Gwosch, K. C., Pape, J. K., Balzarotti, F., Hoess, P., Ellenberg, J., Ries, J., Hell, S. W. MINFLUX nanoscopy delivers multicolor nanometer 3D-resolution in (living) cells.  (bioRxiv, doi: https://doi.org/10.1101/734251)

 

 

 

Event Organizer: Dr. Michael Geller

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