LMI Seminar: Extending the capabilities of multi photon microscopy: continuous volumetric imaging and improved resolution

Pablo Blinder, PhD, Neurobiology, Biochemistry and Biophysics School, George S. Wise Faculty of Life Sciences and Sagol School for Neuroscience, TAU

01 July 2020, 13:00 
Zoom: zoom.us/j/97033684693 
LMI Seminar

Zoom: zoom.us/j/97033684693

 

Abstract:

Multi-photon microscopy exploits the use of NIR, ultrafast light to image deep into scattering media and has become the the gold-standard  for the in vivo, sub-cellular imaging, particularly in neuroscience. The low efficiency of the two (or) three-photon absorption confines fluorescence excitation of a very limited focal volume and requires scanning along the axial plane of interest to generate an image. I will share during the seminar our advances in extending multi photon microscopy from planar to continuous volumetric imaging and the challenges we overcome to increase sampling under photon-deprived conditions.

 

 

 

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